Recycled fibers known as secondary fibers become indispensible raw material for paper industry. In paper industry, recovered paper utilization strongly depends on developments in ink removal process which has rapidly improved. The aim of deinking which is largely a chemical process, is to detach printing inks from the fibers and to remove detached ink particles from the pulp slurry to prevent them redeposition on the fiber surface. Currently, the increase of environmental awareness and energy saving concerns forced paper industry to search a new pursuit as an alternative to chemical deinking. Enzyme applications are among the new technologies that can help the removal of ink to take into considiration of these actual concerns. Even enzymes have been used in the paper industry for various purposes for many years, it is new to use enzymes in deinking applications. Altough the exact mechanism of enzymes is stil unknown, it is certain that enzymes change fibre properties. This study presents how enzymes work to remove ink from the fibers and which parameters are important to obtain the best results. It is clear that along with the positive results in laboratory studies, the use of enzymes in industry will increase and as a result the enzyme costs will drop to much more reasonable levels.