In this study, the effect of seedbed density on the field performance of Anatolian black pine seedlings was investigated. Seeds from Afyon-Ahırdağı provenance were sown in seedbeds which had seven sowing rows with 15 cm spacing between rows in Eskişehir Forest Nursery in 2002. Seedlings grown with seedbed densities of control (1.0cm), 1.5 cm, 2.5 cm, 5.0 cm, 7.5 cm, and 10.0 cm spacings in the nursery were planted with 3.0x1.5 m spacing as 2+0 year-old in Kırka-Eskişehir region. Survivals, height, and root collar diameter of the seedlings were measured at the end of the 5th and 8th vegetation periods after planting. All data were evaluated by analysis of variance and Duncan’s test. As a result, seedling grown with 10 cm seedbed density in the nursery showed the best growth at the end of the 8th vegetation period. But, considering the need for producing the most amount of plantable seedling, establishment period of the seedlings, and differences in height of the seedlings at the end of the 8th vegetation period after planting, it can be concluded that producing 250 seedling per square meter was more suitable in the nursery.